MAHE Manipal B.Sc Nursing 2025
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Polymerase Chain Reaction is considered one of the most asked concept.
39 Questions around this concept.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
1 ) Artificially synthesised
2) 100 -200 nucleotide long generally
3) single-stranded
4) Complementary to target mRNA
5) It can be genomic DNA or cDNA
From the above statements, select the correct statement for the properties of the primer
Match the following:
A) Denaturation |
1) The reaction is cooled down to 55 to 65 degrees Celsius |
B) Annealing |
2) temp of the reaction is raised to 72 degrees Celsius |
C) Extension |
3) The reaction is heated at 94 degrees Celsius |
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Which one is a true statement regarding DNA polymerase used in PCR
Stages in PCR:
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