Vectorless gene transfer - Practice Questions & MCQ

Microinjection:

• In this method, the foreign DNA is directly injected into the nucleus of an animal cell or plant cell by using microneedles or micropipettes.
• It is used in oocytes and embryos. 
• The term transfection is used for the transfer of DNA into eukaryotic cells, by various physical or chemical means.

Biolistics or Gene-gun:

• Biolistics is a means of introducing DNA into cells that involve the bombardment of cells with high-velocity microprojectiles coated with DNA.
• In this method, tungsten or gold particles, coated with foreign DNA are bombarded into target cells at a very high rate.
• It is extensively used for plant cells.