Starting from the roots to aerial parts of the plant the upward transport of water is called ascent of sap.
In the plants, the process occurs through xylem tissue.
This was proved through two sets of experiments:
The cut end of the shoot is immersed in a solution containing eosin or safranin dye. As the water is absorbed, the xylem elements got coloured. It was demonstrated by Stephan Hales.
Ringing Experiment: It was demonstrated by Malpighi with a focus on phloem and Stephan Hales with a focus on xylem. A ring of bark was removed from the stem. This removed phloem from the stem and stopped food conduction. However, water conduction continued. Accumulation of the food caused swelling in the cut region.
There are three types of theories to explain the ascent of sap: