Bioassay for Gibberellin MCQ - Practice Questions with Answers

Dwarf pea bioassay

• Seeds of dwarf peas are allowed to germinate till the formation of coleoptile. 
• GA solution is applied to some seedlings. 
• Others are kept as control. 
• After 5 days, epicotyl length is measured. 
• GA stimulates epicotyl growth with a concentration as low as 1 Nanogram.

Barley endosperm bioassay

• Endosperms are detached from embryos, sterilized and allowed to remain in 1 ml of test solution for 1-2 days. 
• There is a build-up of reducing sugars. 
• The content of reducing sugar is proportional to gibberellin concentration. 
• Reduc­ing sugars are not formed in control experiment where endosperms are kept in plain water.