- In this method, the plants are propagated by culturing the cells, tissues, and organs.
Methods of Micro-Propagation:
1. Multiplication by Meristematic Tissue of Axillary and Apical Shoots:
- Axillary and apical shoots contain quiescent or active meristems depending on the physiological state of the plant.
- When these shoot tips are cultured on a basal medium containing no growth regulators, these typically develop into shoots.
2. Multiplication by Adventitious Embryo Formation:
- Adventitious embryos can arise directly from a group of cells within the original explants or from primary embryoids.
- These adventitious embryoids are diploid in nature and can be used as clonal material for micro-propagation.
3. Multiplication through Callus Culture:
- Direct plantlet formation from the explants in culture is more desirable in case of micropropagation or clonal propagation.
- But shoot formation may occur through organogenesis or embryogenesis from callus produced from the explant.