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RNAi Technology MCQ - Practice Questions with Answers

Edited By admin | Updated on Sep 18, 2023 18:34 AM | #NEET

Quick Facts

  • Pest Resistant Transgenic Plant Using RNAi is considered one of the most asked concept.

  • 3 Questions around this concept.

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Pest Resistant Transgenic Plant Using RNAi
  • Many nematodes live in plants and animals including human beings. 
  • A nematode Meloidogyne incognita infects the roots of tobacco plants and causes a great reduction in yield. 
  • A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi). 
  • RNAi takes place in all eukaryotic organisms as a method of cellular defence. 
  • This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).
  • The source of this complementary RNA could be from infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.
  • Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant.
  • The introduction of DNA was such that it produced both sense and antisense RNA in the host cells.
  •  These two RNA’s being complementary to each other formed a double-stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode.
  • The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA.
  • The transgenic plant, therefore, got itself protected from the parasite.
     

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