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RNAi Technology - Practice Questions & MCQ
Edited By admin | Updated on Sep 18, 2023 18:34 AM | #NEET
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Pest Resistant Transgenic Plant Using RNAi is considered one of the most asked concept.
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Pest Resistant Transgenic Plant Using RNAi
- Many nematodes live in plants and animals including human beings.
- A nematode Meloidogyne incognita infects the roots of tobacco plants and causes a great reduction in yield.
- A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference (RNAi).
- RNAi takes place in all eukaryotic organisms as a method of cellular defence.
- This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).
- The source of this complementary RNA could be from infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.
- Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant.
- The introduction of DNA was such that it produced both sense and antisense RNA in the host cells.
- These two RNA’s being complementary to each other formed a double-stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode.
- The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA.
- The transgenic plant, therefore, got itself protected from the parasite.
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